Protective effects of manganese(II) chloride on hyaluronan degradation by oxidative system ascorbate plus cupric chloride

The degradation of several high-molar-mass hyaluronan samples was investigated in the presence of ascorbic acid itself and further by an oxidative system composed of ascorbic acid plus transition metal ions, i.e. Fe(II) or Cu(II) ions. The latter oxidative system imitates conditions in a joint synovial fluid during early phase of acute joint inflammation and can be used as a model for monitoring oxidative degradation of hyaluronan under pathophysiological conditions. The system Cu(II) plus ascorbate (the Weissberger oxidative system) resulted in a more significant decrease of hyaluronan molar mass compared to the oxidative system Fe(II) plus ascorbate. Addition of manganese(II) chloride was found to decrease the rate of the oxidative damage of hyaluronan initiated by ascorbate itself and by the Weissberger system.